#!/home/passala/miniconda3/envs/Genomealign/bin/perl =head1 NAME bp_index.pl - indexes files for use by bp_fetch.pl =head1 SYNOPSIS bp_index.pl index_name file1 file2 etc. =head1 DESCRIPTION bp_index.pl builds a bioperl index for the sequence files given in the argument list, under the index name. For example bp_index.pl nrdb /data/nrdb/nrdb.fasta would build an index called 'nrdb' as the index name for the file nrdb.fasta, and bp_index.pl -fmt EMBL swiss /data/swiss/*.dat would build an index called swiss for all the files in /data/swiss which end in .dat which are in EMBL format. The indexes are built using the Bio/Index/* modules, in particular, Bio::Index::EMBL and the Bio::Index::Fasta modules. Any script which uses these modules can use the index. A good example script is bp_fetch which fetches sequences and pipes them to STDOUT, for example bp_fetch swiss:ROA1_HUMAN gets the ROA1_HUMAN sequence from the swiss index and writes it as fasta format on STDOUT. =head1 OPTIONS -fmt - Fasta (default), swiss or EMBL -dir - directory where the index files are found (overrides BIOPERL_INDEX environment variable) Options for expert use -type - DBM_file type. (overrides BIOPERL_INDEX_TYPE environment variable) -v - report every index addition (debugging) =head1 ENVIRONMENT bp_index and bp_fetch coordinate where the databases lie using the environment variable BIOPERL_INDEX. This can be overridden using the -dir option. There is no default value, so you must use the -dir option or set BIOPERL_INDEX. The DB type is coordinated with BIOPERL_INDEX_TYPE which if it is not there, defaults to whatever the bioperl modules have installed, which itself defaults to SDBM_File. =head1 USING IT YOURSELF bp_index.pl is a script that drives the Index modules. If you want to use this script heavily in your work, if it is Perl based, it is almost certainly better to look at the code in this script and copy it across (probably you will be more likely to want to use the bp_fetch code). =head1 EXTENDING IT bp_index is just a wrapper around James Gilbert's excellent Index modules found in bioperl =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: https://github.com/bioperl/bioperl-live/issues =head1 AUTHOR - Ewan Birney Ewan Birney Ebirney@ebi.ac.ukE =cut #' use strict; use warnings; # # Doofus catcher for people who are trying this script without # installing bioperl # use Bio::Index::Fasta; use Bio::Index::EMBL; use Bio::Index::Swissprot; use Bio::Index::GenBank; use Bio::Index::SwissPfam; my $dir = $ENV{'BIOPERL_INDEX'}; my $type = $ENV{'BIOPERL_INDEX_TYPE'}; my $fmt = 'Fasta'; my $verbose = 0; use Getopt::Long; &GetOptions("f|fmt=s" => \$fmt, "d|dir=s" => \$dir, "t|type=s" => \$type, "v!" => \$verbose); exec('perldoc',$0) unless @ARGV; my $name = shift; if( !$dir ) { print STDERR "\nNo directory specified for index\nDirectory must be specified by the environment variable BIOPERL_INDEX or -dir option\ngo bp_index with no arguments for more help\n\n"; exit(1); } # # Reset the type if needed # if( $type ) { $Bio::Index::Abstract::USE_DBM_TYPE = $type; } # # Rock and roll... # my $index; $_ = $fmt; SWITCH : { /Fasta/i && do { $index = Bio::Index::Fasta->new("$dir/$name", 'WRITE'); last; }; /EMBL/i && do { $index = Bio::Index::EMBL->new("$dir/$name", 'WRITE'); last; }; /swisspfam|pfam/i && do { $index = Bio::Index::SwissPfam->new("$dir/$name", 'WRITE'); last; }; /swiss/i && do { $index = Bio::Index::Swissprot->new("$dir/$name", 'WRITE'); last; }; /GenBank/i && do { $index = Bio::Index::GenBank->new("$dir/$name", 'WRITE'); last; }; die("No index format called $fmt"); } if( $verbose != 0 ) { $index->verbose(1); } $index->make_index(@ARGV); # finished. Neat eh. # # if you are using this in a script, to # to force deallocation + closing of the index, go # $index = undef; #