#!/home/passala/miniconda3/envs/Genomealign/bin/perl =head1 NAME bp_search2table - turn SearchIO parseable reports into tab delimited format like NCBI's -m 9 =head1 SYNOPSIS bp_search2table -f fasta -i file.FASTA -o output.table =head1 DESCRIPTION Turn SearchIO reports into a tabular format like NCBI's -m 9 output. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via email or the web: https://github.com/bioperl/bioperl-live/issues =head1 AUTHOR Jason Stajich jason_at_bioperl-dot-org =cut use strict; use warnings; use Bio::SearchIO; use Getopt::Long; my ($format, $file,$output) = ('blast'); GetOptions( 'f|format:s' => \$format, 'i|input:s' => \$file, 'o|output:s' => \$output); if( @ARGV ) { $file = shift; } my $in = Bio::SearchIO->new(-format => $format, -file => $file); my $out; if( $output ) { open $out, '>', $output or die "Could not write file '$output': $!\n"; } else { $out = \*STDOUT; } while( my $r = $in->next_result ) { while( my $hit = $r->next_hit ) { while( my $hsp = $hit->next_hsp ) { my $mismatchcount = $hsp->length('total') - ($hsp->num_conserved + $hsp->gaps('total')); print $out join("\t", ( $r->query_name, $hit->name, sprintf("%.2f",$hsp->percent_identity), $hsp->length('total'), $mismatchcount, $hsp->gaps('total'), # flip start/end on rev strand $hsp->query->strand < 0 ? ( $hsp->query->end, $hsp->query->start ) : ( $hsp->query->start, $hsp->query->end ), $hsp->hit->strand < 0 ? ( $hsp->hit->end, $hsp->hit->start ) : ( $hsp->hit->start, $hsp->hit->end ), $hsp->evalue, # chance this to $hsp->sw_score # if you would rather have that # it will only work for FASTA parsing though! $hsp->bits)),"\n"; } } }