/*=========================================================================== * * PUBLIC DOMAIN NOTICE * National Center for Biotechnology Information * * This software/database is a "United States Government Work" under the * terms of the United States Copyright Act. It was written as part of * the author's official duties as a United States Government employee and * thus cannot be copyrighted. This software/database is freely available * to the public for use. The National Library of Medicine and the U.S. * Government have not placed any restriction on its use or reproduction. * * Although all reasonable efforts have been taken to ensure the accuracy * and reliability of the software and data, the NLM and the U.S. * Government do not and cannot warrant the performance or results that * may be obtained by using this software or data. The NLM and the U.S. * Government disclaim all warranties, express or implied, including * warranties of performance, merchantability or fitness for any particular * purpose. * * Please cite the author in any work or product based on this material. * * =========================================================================== * */ #include #include #include #include #include #include #include #include #include using namespace ngs; using namespace std; class PileupTest { public: static void run ( String acc, String refname, int start, int stop ) { // open requested accession using SRA implementation of the API ReadCollection run = ncbi::NGS::openReadCollection ( acc ); String run_name = run.getName (); // get requested reference Reference ref = run.getReference ( refname ); // start iterator on requested range long count = stop - start + 1; PileupIterator it = ref.getPileupSlice ( start-1 /*0-based*/, count); long i; for ( i = 0; it . nextPileup (); ++ i ) { String qual; String base; cout << it.getReferenceSpec () << '\t' << ( it.getReferencePosition () + 1 ) << '\t' << it.getReferenceBase () << '\t' << it.getPileupDepth (); while(it.nextPileupEvent()) { PileupEvent::PileupEventType e = it.getEventType (); //cout << e <<'\n'; if(e & PileupEvent::alignment_start) { base += '^'; base += (char) (it.getMappingQuality() + 33 ); } if(e & PileupEvent::insertion) { base += '+'; StringRef ibases= it.getInsertionBases(); int c = ibases.size(); char buf[64]; if(e & PileupEvent::alignment_minus_strand) { char *b = buf + sprintf(buf,"%d",c); const char *s = ibases.data(); for(int i=0; i