FastQCFastQC Report
Tue 13 Jun 2023
12950_A5_S18_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename12950_A5_S18_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83704230
Total Bases12.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCT1363360.16287826792027116No Hit
GGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGT1050990.1255599627402343No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT1022200.1221204710920822No Hit
GGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGG998590.11929982511039168No Hit
GGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGG903500.10793958680463342No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG845780.10104387794977626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)