FastQCFastQC Report
Tue 13 Jun 2023
12950_B3_S24_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename12950_B3_S24_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95820085
Total Bases14.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC5149800.5374447330118732No Hit
CCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC3511110.36642735184382275No Hit
GGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCAT1418500.1480378565725547No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1330920.13889781041208635No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG1214370.1267343897680742No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1116720.11654341571498293No Hit
CAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAA965760.10078888992845289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)