# CMD line shows the command line used to generate this output # DEF lines define expressions for all tested thresholds # FLT* lines report numbers for every threshold and every trio: # 1) filter id # 2) child # 3) father # 4) mother # 5) number of valid trio genotypes (all trio members pass filters, all non-missing) # 6) number of non-reference trio GTs (at least one trio member carries an alternate allele) # 7) number of DNMs/Mendelian errors # 8) number of novel singleton alleles in the child (counted also as DNM / Mendelian error) # 9) number of untransmitted trio singletons (one alternate allele present in one parent) # 10) number of transmitted trio singletons (one alternate allele present in one parent and the child) # 11) number of transitions, all distinct ALT alleles present in the trio are considered # 12) number of transversions, all distinct ALT alleles present in the trio are considered # 13) overall ts/tv, all distinct ALT alleles present in the trio are considered # 14) number of homozygous DNMs/Mendelian errors (likely genotyping errors) # 15) number of recurrent DNMs/Mendelian errors (non-inherited alleles present in other samples; counts GTs, not sites) MERR 20 303 child1 father1 mother1 - RECURRENT MERR 20 304 child1 father1 mother1 HOM RECURRENT MERR 20 305 child1 father1 mother1 HOM - TRANSMITTED 20 306 child1 father1 mother1 NO TRANSMITTED 20 308 child2 father2 mother2 YES TRANSMITTED 20 308 child2 father2 mother2 NO MERR 20 309 child1 father1 mother1 - - TRANSMITTED 20 310 child1 father1 mother1 NO TRANSMITTED 20 311 child2 father2 mother2 YES TRANSMITTED 20 312 child1 father1 mother1 YES DEF FLT0 all FLT0 child1 father1 mother1 14 12 4 2 2 1 3 9 0.33 2 2 FLT0 child2 father2 mother2 14 11 0 0 1 2 3 9 0.33 0 0