/* add_header.c -- showcases the htslib api usage Copyright (C) 2023 Genome Research Ltd. Author: Vasudeva Sarma Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE */ /* The pupose of this code is to demonstrate the library apis and need proper error handling and optimization */ #include #include #include /// print_usage - print the demo_usage /** @param fp pointer to the file / terminal to which demo_usage to be dumped returns nothing */ static void print_usage(FILE *fp) { fprintf(fp, "Usage: add_header infile\n\ Adds new header lines of SQ, RG, PG and CO typs\n"); return; } /// main_demo - start of the demo /** @param argc - count of arguments * @param argv - pointer to array of arguments returns 1 on failure 0 on success */ int main(int argc, char *argv[]) { const char *inname = NULL, sq[] = "@SQ\tSN:TR1\tLN:100\n@SQ\tSN:TR2\tLN:50"; int c = 0, ret = EXIT_FAILURE; samFile *infile = NULL, *outfile = NULL; sam_hdr_t *in_samhdr = NULL; kstring_t data = KS_INITIALIZE; //update_header infile header idval tag value if (argc != 2) { print_usage(stderr); goto end; } inname = argv[1]; if (!(infile = sam_open(inname, "r"))) { printf("Could not open %s\n", inname); goto end; } if (!(outfile = sam_open("-", "w"))) { //use stdout as the output file for ease of display of update printf("Could not open stdout\n"); goto end; } //read header if (!(in_samhdr = sam_hdr_read(infile))) { printf("Failed to read header from file!\n"); goto end; } //dump command line arguments for PG line for (c = 0; c < argc; ++c) { kputs(argv[c], &data); kputc(' ', &data); } //add SQ line with SN as TR1 and TR2 if (sam_hdr_add_lines(in_samhdr, &sq[0], 0)) { //length as 0 for NULL terminated data printf("Failed to add SQ lines\n"); goto end; } //add RG line with ID as RG1 if (sam_hdr_add_line(in_samhdr, "RG", "ID", "RG1", "LB", "Test", "SM", "S1", NULL)) { printf("Failed to add RG line\n"); goto end; } //add pg line if (sam_hdr_add_pg(in_samhdr, "add_header", "VN", "Test", "CL", data.s, NULL)) { //NULL is to indicate end of args printf("Failed to add PG line\n"); goto end; } if (sam_hdr_add_line(in_samhdr, "CO", "Test data", NULL)) { //NULL is to indicate end of args printf("Failed to add PG line\n"); goto end; } //write output if (sam_hdr_write(outfile, in_samhdr) < 0) { printf("Failed to write output\n"); goto end; } ret = EXIT_SUCCESS; //bam data write to follow.... end: //cleanup if (in_samhdr) { sam_hdr_destroy(in_samhdr); } if (infile) { sam_close(infile); } if (outfile) { sam_close(outfile); } ks_free(&data); return ret; }