/* flags_demo.c -- showcases the htslib api usage Copyright (C) 2023 Genome Research Ltd. Author: Vasudeva Sarma Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE */ /* The pupose of this code is to demonstrate the library apis and need proper error handling and optimization */ #include #include #include /// print_usage - show flags_demo usage /** @param fp pointer to the file / terminal to which demo_usage to be dumped returns nothing */ static void print_usage(FILE *fp) { fprintf(fp, "Usage: flags \n\ Shows the count of read1 and read2 alignments\n\ This shows basic reading and alignment flag access\n"); return; } /// main_demo - start of the demo /** @param argc - count of arguments * @param argv - pointer to array of arguments returns 1 on failure 0 on success */ int main(int argc, char *argv[]) { const char *inname = NULL; //input file name int c = 0, ret = EXIT_FAILURE; int64_t cntread1 = 0, cntread2 = 0; //count samFile *infile = NULL; //sam file sam_hdr_t *in_samhdr = NULL; //header of file bam1_t *bamdata = NULL; //to hold the read data if (argc != 2) { print_usage(stdout); goto end; } inname = argv[1]; //initialize if (!(bamdata = bam_init1())) { printf("Failed to initialize bamdata\n"); goto end; } //open input files - r reading if (!(infile = sam_open(inname, "r"))) { printf("Could not open %s\n", inname); goto end; } //read header if (!(in_samhdr = sam_hdr_read(infile))) { printf( "Failed to read header from file\n"); goto end; } //read data, check flags and update count while ((c = sam_read1(infile, in_samhdr, bamdata)) >= 0) { if (bamdata->core.flag & BAM_FREAD1) { cntread1++; } if (bamdata->core.flag & BAM_FREAD2) { cntread2++; } } if (c != -1) { //error printf("Failed to get data\n"); goto end; } //else -1 / EOF printf("File %s has %"PRIhts_pos" read1 and %"PRIhts_pos" read2 alignments\n", inname, cntread1, cntread2); ret = EXIT_SUCCESS; end: //clean up if (in_samhdr) { sam_hdr_destroy(in_samhdr); } if (infile) { sam_close(infile); } if (bamdata) { bam_destroy1(bamdata); } return ret; }