/* index_multireg_read.c -- showcases the htslib api usage Copyright (C) 2023 Genome Research Ltd. Author: Vasudeva Sarma Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE */ /* The pupose of this code is to demonstrate the library apis and need proper error handling and optimization */ #include #include #include /// print_usage - print the print_usage /** @param fp pointer to the file / terminal to which print_usage to be dumped returns nothing */ static void print_usage(FILE *fp) { fprintf(fp, "Usage: read_multireg infile count regspec_csv\n\ Reads alignment of a target matching to given region specifications\n\ read_multireg infile.sam 2 R1:10-100,R2:200"); return; } /// main_demo - start of the demo /** @param argc - count of arguments * @param argv - pointer to array of arguments returns 1 on failure 0 on success */ int main(int argc, char *argv[]) { const char *inname = NULL; char *ptr = NULL; int c = 0, ret = EXIT_FAILURE; samFile *infile = NULL, *outfile = NULL; sam_hdr_t *in_samhdr = NULL; bam1_t *bamdata = NULL; hts_idx_t *idx = NULL; hts_itr_t *iter = NULL; unsigned int regcnt = 0; char **regions = NULL; //read_multireg infile count regspec_csv if (argc != 4) { print_usage(stderr); goto end; } inname = argv[1]; regcnt = atoi(argv[2]); regions = calloc(regcnt, sizeof(char*)); //set each regspec as separate entry in region array ptr = argv[3]; for (c = 0; ptr && (c < regcnt); ++c) { regions[c] = ptr; ptr = strchr(ptr, ','); if (ptr) { *ptr = '\0'; ++ptr; } } if (regcnt == 0) { printf("Region count can not be 0\n"); goto end; } //initialize bam data storage if (!(bamdata = bam_init1())) { printf("Failed to initialize bamdata\n"); goto end; } //open files, use stdout as output SAM file for ease of display infile = sam_open(inname, "r"); outfile = sam_open("-", "w"); if (!outfile || !infile) { printf("Could not open in/out files\n"); goto end; } //load index file, assume it to be present in same location if (!(idx = sam_index_load(infile, inname))) { printf("Failed to load the index\n"); goto end; } //read header if (!(in_samhdr = sam_hdr_read(infile))) { printf("Failed to read header from file!\n"); goto end; } //create iterator if (!(iter = sam_itr_regarray(idx, in_samhdr, regions, regcnt))) { printf("Failed to get iterator\n"); goto end; } if (regions) { //can be freed as it is no longer required free(regions); regions = NULL; } //get required area while ((c = sam_itr_multi_next(infile, iter, bamdata) >= 0)) { //write to output if (sam_write1(outfile, in_samhdr, bamdata) < 0) { printf("Failed to write output\n"); goto end; } } if (c != -1) { printf("Error during read\n"); goto end; } ret = EXIT_SUCCESS; end: //cleanup if (in_samhdr) { sam_hdr_destroy(in_samhdr); } if (infile) { sam_close(infile); } if (outfile) { sam_close(outfile); } if (bamdata) { bam_destroy1(bamdata); } if (iter) { sam_itr_destroy(iter); } if (idx) hts_idx_destroy(idx); return ret; }