/* index_write.c -- showcases the htslib api usage Copyright (C) 2023 Genome Research Ltd. Author: Vasudeva Sarma Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE */ /* The pupose of this code is to demonstrate the library apis and need proper error handling and optimization */ #include #include #include #include /// print_usage - print the demo_usage /** @param fp pointer to the file / terminal to which demo_usage to be dumped returns nothing */ static void print_usage(FILE *fp) { fprintf(fp, "Usage: idx_on_write infile shiftsize outdir\n\ Creates compressed sam file and index file for it in given directory\n"); return; } /// main_demo - start of the demo /** @param argc - count of arguments * @param argv - pointer to array of arguments returns 1 on failure 0 on success */ int main(int argc, char *argv[]) { const char *outdir = NULL; char *inname = NULL, *fileidx = NULL, *outname = NULL, outmode[4] = "w"; int c = 0, ret = EXIT_FAILURE, size = 0; samFile *infile = NULL, *outfile = NULL; sam_hdr_t *in_samhdr = NULL; bam1_t *bamdata = NULL; //idx_on_write infile sizeshift outputdirectory if (argc != 4) { print_usage(stderr); goto end; } inname = argv[1]; size = atoi(argv[2]); outdir = argv[3]; //allocate space for output name - outdir/filename.ext.idxextNUL c = strlen(basename(inname)) + strlen(outdir) + 10; fileidx = malloc(sizeof(char) * c); outname = malloc(sizeof(char) * c); if (!fileidx || !outname) { printf("Couldnt allocate memory\n"); goto end; } //initialize bam storage if (!(bamdata = bam_init1())) { printf("Failed to initialize bamdata\n"); goto end; } //open files if ((infile = sam_open(inname, "r"))) { //get file type and create output names if (infile->format.format == cram) { //set as crai snprintf(fileidx, c, "%s/%s.crai", outdir, basename(inname)); snprintf(outname, c, "%s/%s", outdir, basename(inname)); } else { //set as either bai or csi based on interval if (infile->format.format == sam && infile->format.compression == no_compression) { //create as gzip compressed snprintf(outname, c, "%s/%s.gz", outdir, basename(inname)); snprintf(fileidx, c, "%s/%s.gz.%s", outdir, basename(inname), !size ? "bai" : "csi"); } else { //with same name as input snprintf(outname, c, "%s/%s", outdir, basename(inname)); snprintf(fileidx, c, "%s/%s.%s", outdir, basename(inname), !size ? "bai" : "csi"); } } } c = 0; sam_open_mode(outmode + 1, outname, NULL); //set extra write options based on name outfile = sam_open(outname, outmode); if (!outfile || !infile) { printf("Could not open files\n"); goto end; } //read header if (!(in_samhdr = sam_hdr_read(infile))) { printf("Failed to read header from file!\n"); goto end; } //write header if (sam_hdr_write(outfile, in_samhdr)) { printf("Failed to write header\n"); goto end; } // initialize indexing, before start of write if (sam_idx_init(outfile, in_samhdr, size, fileidx)) { printf("idx initialization failed\n"); goto end; } //read and write alignments while ((c = sam_read1(infile, in_samhdr, bamdata)) >= 0) { if (sam_write1(outfile, in_samhdr, bamdata) < 0) { printf("Failed to write data\n"); goto end; } } if (c != -1) { printf("Error in reading data\n"); goto end; } //else EOF, save index if (sam_idx_save(outfile)) { printf("Could not save index\n"); goto end; } ret = EXIT_SUCCESS; end: //cleanup if (in_samhdr) { sam_hdr_destroy(in_samhdr); } if (infile) { sam_close(infile); } if (bamdata) { bam_destroy1(bamdata); } if (fileidx) { free(fileidx); } if (outname) { free(outname); } if (outfile) { sam_close(outfile); } return ret; }