/* read_refname.c -- showcases the htslib api usage Copyright (C) 2023 Genome Research Ltd. Author: Vasudeva Sarma Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE */ /* The pupose of this code is to demonstrate the library apis and need proper error handling and optimization */ #include #include #include /// print_usage - print the demo_usage /** @param fp pointer to the file / terminal to which demo_usage to be dumped returns nothing */ static void print_usage(FILE *fp) { fprintf(fp, "Usage: read_refname infile minsize\n\ This shows name of references which has length above the given size\n"); return; } /// main_demo - start of the demo /** @param argc - count of arguments * @param argv - pointer to array of arguments returns 1 on failure 0 on success */ int main(int argc, char *argv[]) { const char *inname = NULL, *id = NULL; int c = 0, ret = EXIT_FAILURE, linecnt = 0, pos = 0; samFile *infile = NULL; sam_hdr_t *in_samhdr = NULL; kstring_t data = KS_INITIALIZE; int64_t minsize = 0, size = 0; if (argc != 3 && argc != 2) { print_usage(stdout); goto end; } inname = argv[1]; if (argc == 3) { minsize = atoll(argv[2]); } //open input files if (!(infile = sam_open(inname, "r"))) { printf("Could not open %s\n", inname); goto end; } //read header if (!(in_samhdr = sam_hdr_read(infile))) { printf("Failed to read header from file!\n"); goto end; } linecnt = sam_hdr_count_lines(in_samhdr, "SQ"); //get reference count if (linecnt <= 0) { if (!linecnt) { printf("No reference line present\n"); } else { printf("Failed to get reference line count\n"); } goto end; } //iterate and check each reference's length for (pos = 1, c = 0; c < linecnt; ++c) { if ((ret = sam_hdr_find_tag_pos(in_samhdr, "SQ", c, "LN", &data) == -2)) { printf("Failed to get length\n"); goto end; } else if (ret == -1) { //length not present, ignore continue; } //else have length size = atoll(data.s); if (size < minsize) { //not required continue; } if (!(id = sam_hdr_line_name(in_samhdr, "SQ", c))) { //sam_hdr_find_tag_pos(in_samhdr, "SQ", c, "SN", &data) can also do the same! printf("Failed to get id for reference data\n"); goto end; } printf("%d,%s,%s\n", pos, id, data.s); pos++; } ret = EXIT_SUCCESS; end: //cleanup if (in_samhdr) { sam_hdr_destroy(in_samhdr); } if (infile) { sam_close(infile); } ks_free(&data); return ret; }