/* Copyright (C) 2017, 2020, 2023 Genome Research Ltd. Author: Petr Danecek Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ /* Test bcf synced reader allele pairing */ #include #include #include #include #include #include #include #include #include "../htslib/synced_bcf_reader.h" #include "../htslib/hts.h" #include "../htslib/vcf.h" void error(const char *format, ...) { va_list ap; va_start(ap, format); vfprintf(stderr, format, ap); va_end(ap); exit(EXIT_FAILURE); } void usage(int exit_code) { fprintf(stderr, "Usage: test-bcf-sr [OPTIONS] vcf-list.txt\n"); fprintf(stderr, " test-bcf-sr [OPTIONS] -args file1.bcf [...]\n"); fprintf(stderr, "Options:\n"); fprintf(stderr, " --args pass filenames directly in argument list\n"); fprintf(stderr, " --no-index allow streaming\n"); fprintf(stderr, " -o, --output output file (stdout if not set)\n"); fprintf(stderr, " -O, --output-fmt fmt: vcf,bcf,summary\n"); fprintf(stderr, " -p, --pair logic: snps,indels,both,snps+ref,indels+ref,both+ref,exact,some,all\n"); fprintf(stderr, " -r, --regions comma-separated list of regions\n"); fprintf(stderr, " -t, --targets comma-separated list of targets\n"); fprintf(stderr, "\n"); exit(exit_code); } void write_summary_format(bcf_srs_t *sr, FILE *out) { int n, i, j; while ((n = bcf_sr_next_line(sr)) > 0) { for (i=0; inreaders; i++) { if ( !bcf_sr_has_line(sr,i) ) continue; bcf1_t *rec = bcf_sr_get_line(sr, i); if (!rec) error("bcf_sr_get_line() unexpectedly returned NULL\n"); fprintf(out, "%s:%"PRIhts_pos, bcf_seqname_safe(bcf_sr_get_header(sr,i),rec),rec->pos+1); break; } for (i=0; inreaders; i++) { fprintf(out, "\t"); if ( !bcf_sr_has_line(sr,i) ) { fprintf(out, "%s","-"); continue; } bcf1_t *rec = bcf_sr_get_line(sr, i); if (!rec) error("bcf_sr_get_line() unexpectedly returned NULL\n"); fprintf(out, "%s", rec->n_allele > 1 ? rec->d.allele[1] : "."); for (j=2; jn_allele; j++) { fprintf(out, ",%s", rec->d.allele[j]); } } fprintf(out, "\n"); } } void write_vcf_bcf_format(bcf_srs_t *sr, bcf_hdr_t *hdr, vcfFile *vcf_out, const char *fmt_type) { int i, n; if (bcf_hdr_write(vcf_out, hdr) != 0) error("Couldn't write %s header\n", fmt_type); while ((n = bcf_sr_next_line(sr)) > 0) { for (i=0; inreaders; i++) { if ( !bcf_sr_has_line(sr,i) ) continue; bcf1_t *rec = bcf_sr_get_line(sr, i); if (!rec) error("bcf_sr_get_line() unexpectedly returned NULL\n"); if (vcf_write(vcf_out, hdr, rec) < 0) error("vcf_write() failed\n"); } } } int main(int argc, char *argv[]) { static struct option loptions[] = { {"help",no_argument,NULL,'h'}, {"output-fmt",required_argument,NULL,'O'}, {"pair",required_argument,NULL,'p'}, {"regions",required_argument,NULL,'r'}, {"targets",required_argument,NULL,'t'}, {"no-index",no_argument,NULL,1000}, {"args",no_argument,NULL,1001}, {NULL,0,NULL,0} }; int c, pair = 0, use_index = 1, use_fofn = 1; enum htsExactFormat out_fmt = text_format; // for original pos + alleles const char *out_fn = NULL, *regions = NULL, *targets = NULL; while ((c = getopt_long(argc, argv, "o:O:p:r:t:h", loptions, NULL)) >= 0) { switch (c) { case 'o': out_fn = optarg; break; case 'O': if (!strcasecmp(optarg, "vcf")) out_fmt = vcf; else if (!strcasecmp(optarg, "bcf")) out_fmt = bcf; else if (!strcasecmp(optarg, "summary")) out_fmt = text_format; else error("Unknown output format \"%s\"\n", optarg); break; case 'p': if ( !strcmp(optarg,"snps") ) pair |= BCF_SR_PAIR_SNPS; else if ( !strcmp(optarg,"snp+ref") ) pair |= BCF_SR_PAIR_SNPS|BCF_SR_PAIR_SNP_REF; else if ( !strcmp(optarg,"snps+ref") ) pair |= BCF_SR_PAIR_SNPS|BCF_SR_PAIR_SNP_REF; else if ( !strcmp(optarg,"indels") ) pair |= BCF_SR_PAIR_INDELS; else if ( !strcmp(optarg,"indel+ref") ) pair |= BCF_SR_PAIR_INDELS|BCF_SR_PAIR_INDEL_REF; else if ( !strcmp(optarg,"indels+ref") ) pair |= BCF_SR_PAIR_INDELS|BCF_SR_PAIR_INDEL_REF; else if ( !strcmp(optarg,"both") ) pair |= BCF_SR_PAIR_BOTH; else if ( !strcmp(optarg,"both+ref") ) pair |= BCF_SR_PAIR_BOTH_REF; else if ( !strcmp(optarg,"any") ) pair |= BCF_SR_PAIR_ANY; else if ( !strcmp(optarg,"all") ) pair |= BCF_SR_PAIR_ANY; else if ( !strcmp(optarg,"some") ) pair |= BCF_SR_PAIR_SOME; else if ( !strcmp(optarg,"exact") ) pair = BCF_SR_PAIR_EXACT; else error("The --pair logic \"%s\" not recognised.\n", optarg); break; case 'r': regions = optarg; break; case 't': targets = optarg; break; case 1000: use_index = 0; break; case 1001: use_fofn = 0; break; case 'h': usage(EXIT_SUCCESS); default: usage(EXIT_FAILURE); } } if ( !pair ) pair = BCF_SR_PAIR_EXACT; if ( optind == argc ) usage(EXIT_FAILURE); int i, nvcf; char **vcfs = NULL; if (use_fofn) { vcfs = hts_readlist(argv[optind], 1, &nvcf); if ( !vcfs ) error("Could not parse %s\n", argv[optind]); } else { vcfs = &argv[optind]; nvcf = argc - optind; } bcf_srs_t *sr = bcf_sr_init(); if (!sr) error("bcf_sr_init() failed\n"); bcf_sr_set_opt(sr, BCF_SR_PAIR_LOGIC, pair); if (use_index) { bcf_sr_set_opt(sr, BCF_SR_REQUIRE_IDX); } else { bcf_sr_set_opt(sr, BCF_SR_ALLOW_NO_IDX); } if (regions) { if (bcf_sr_set_regions(sr, regions, 0) != 0) error("Failed to set regions\n"); } if (targets) { if (bcf_sr_set_targets(sr, targets, 0, 0) != 0) error("Failed to set targets\n"); } for (i=0; ierrnum)); if (!sr->readers || sr->nreaders < 1) error("No readers set, even though one was added\n"); if (out_fmt == text_format) { FILE *out = stdout; if (out_fn) { out = fopen(out_fn, "w"); if (!out) error("Couldn't open \"%s\" for writing: %s\n", out_fn, strerror(errno)); } write_summary_format(sr, out); if (out_fn) { if (fclose(out) != 0) error("Error on closing %s : %s\n", out_fn, strerror(errno)); } } else { const char *fmt_type = out_fmt == vcf ? "VCF" : "BCF"; bcf_hdr_t *hdr = bcf_sr_get_header(sr, 0); if (!hdr) error("%s output, but don't have a header\n", fmt_type); if (!out_fn) { out_fn = "-"; } vcfFile *vcf_out = vcf_open(out_fn, out_fmt == vcf ? "w" : "wb"); if (!vcf_out) error("Couldn't open \"%s\" for writing: %s\n", out_fn, strerror(errno)); write_vcf_bcf_format(sr, hdr, vcf_out, fmt_type); if (vcf_close(vcf_out) != 0) error("Error on closing \"%s\"\n", out_fn); } if (sr->errnum) error("Synced reader error: %s\n", bcf_sr_strerror(sr->errnum)); bcf_sr_destroy(sr); if (use_fofn) { for (i=0; i