Triple mouse M Double glass window, no artificial dura Behaving in Simon's 2-mode detection task Visual expert =========================== 7/30/18 Imaging depth is 200um. Location should match ALM. SNR is pretty bad. We'll see if anything useful comes of it. Red channel has tdTomato tag. See some cells there. Laser power is 50%. Lots of motion. Motion correction might benefit from using red channel instead of green. If it turns out unusable, that might be a good thing to try. First 150 trials are all visual. Switched to 20% audio probability after that. Mouse is not very motivated anymore after 300 performed trials. Switched to all audio - will hopefully get some more. 20000 up 10 35000 up 10 58000 up 5 65000 up 10 80000 up 5 90000 up 15 Bpod crashed after 321 performed trials. Will stop here. Testing laser power after imaging session. Our power meter. Does not seem different from 'cold' measure. 10% 6.5mW 20% 21.4mW 30% 43mW 40% 66mW 50% 93mW 60% 116mW 70% 131mW 80% 138mW 90% 136mW =========================== 8/03/18 PMTs at 0.455 Location should match ALM. Imaging depth is 180um. First 8 trials are at 160um depth, should be rejected from analysis. SNR is not good, some connective tissue over brain. Laser power at 50%. Shielding is good. Quite a bit of movement. Switched to all audio after 360 performed trials. Probability was 90/10%, vision/audio before that. 15000 up 10 23000 up 5 45000 up 5 60000 up 20 - should have moved earlier. 95000 up 5 112000 up 10 =========================== 8/07/18 PMTs at 0.455 Location is MM. Shielding is good, quite some motion. Signal looks ok, some active cells. Depth is 180um At ~20000, the plan shifted a lot in Z. Took a moment to find it again. Luckily the animal didn't do trials at that moment so maybe not that bad. Moved 53 up to get back to starting point. Had to correct even more later. Moving a little back and forth to stick to right plane. Animal doesnt seem motivated. Stopped recording after 100 performed trials. This is not going to work out. =========================== 09/13/18 PMTs at 0.455 Laser at 55%. Location is MM, close to last time. Signal is not very good. See some active cells. Depth is 150um. Quite a bit of movement in this one. Too much movement to record anything. Will run without imaging and check headbar later. =========================== 09/14/18 PMTs at 0.455 Laser at 55%. Location is MM, more medial than last time. Signal is not great. quite a bit of movement and bad SNR. See some cells though. Depth is 180um. First 125 trials are vision only. Added 20% audio after that. Switched to all audio after 475 performed trials. Stopped after 525 trials. Visual performance at 60%. Audio at chance.