Triple mouse M
Double glass window, no artificial dura
Behaving in Simon's 2-mode detection task
Visual expert

5/10/18

Objective at 13.7, slightly angled but should be ok.
Should do better next time.

PMT at 445
Some motion but seems fine.
Task, starting visual trials only.

Using normal LED "house lights"

Imaging at surface1, rougly center of the window
Shielding seems fine.
Depth 250 um
Laser 33%
Continuous imaging

Field is not great but see enough neurons to proceed.
Mainly in the center.
Scanners at 2.5V.


Chasing Z drift:
14000 up 2
30000 up 2
33000 up 1
38000 up 1
43000 up 2
48000 up 2
53000 up 2
55000 up 1
58000 up 1
61300 up 1
65500 up 2
71200 up 2
75900 up 1
79000 up 2
81500 up 1
86500 up 2
91500 up 2
96600 up 2
10300 up 2
11100 down 2
11400 up 2
11700 up 3
11900 up 1
12300 up 2
13000 up 2
13400 up 2
13900 up 3
14500 up 2
14800 up 1
15400 up 2
16000 up 2
lost track of the plane. moved around a little bit and came back to where I was before. Maybe subsequent data makes little sense.
17500 up 2
stopped at 174805

Behavior was not great but ok. Visual ~70%, audio at chance. Got worse towards the end.

===============================================================

5/15/18
depth: 170um
angle: ~10 deg
a little bit of light leak from visual stimuli, maybe some movement but hopefully ok
Signal looks good. Many lick tuned neurons. Best one  so far.

Shielding seems fine.
Depth 170 um
Laser 34%
Continuous imaging


Chasing Z drift:
5000 up 1
1000 up 2
1500 up 2
1800 up 1
2200 up 2
2600 up 1
3000 up 2
3700 up 1
4100 up 2
4600 up 2
5500 up 2
6000 up 2
6200 up 1
6400 up 2
6800 up 1
7200 up 2
7600 up 2
8000 up 2
8400 up 2
9200 up 2
9700 up 2
10000 up 2
10500 down 1
10800 down 2
11500 down 2
drift seems to have stopped/slowed down
Fairly certain that we kept the same cells across the session (maybe except for the part where I had to go down again).

pupil is covered with stuff at ~350 trials
behavior was fine. 75% visual, 50% audio. ~400 trials.


===============================================================

5/17/18
angle: 10.5 deg - still slightly angled. Unfolding from posteriorlateral to anteriormedial.
Should be fine though.
depth: 190um
laser power 34%
location is pretty close to where we imaged last time.
signal looks pretty good - many neurons.

Chasing Z drift:
5000 up 1
10000 up 2
some major noise at around 12000-13000. had to open the door to switch on ambient light.
15000 up 2
18000 up 2
21000 up 2
25000 up 1
29000 up 3
32000 up 2
36000 up 2
42000 up 2
49000 up 2
53000 up 2
62000 up 1
65000 down 2
80000 seems like drift is fairly stable now.

Animal has donly only 150 trials though... squinting a lot. Pupil tracking will presumably be not ideal.
Mostly running on vision only to keep the mouse working. Hopefully we'll get enough trials together.
Behavior is pretty weak, ~66% vision, 50% audio. Only 250 performed trials.