Triple mouse M
Double glass window, no artificial dura
Behaving in Simon's 2-mode detection task
Visual expert

5/10/18

Objective at 13.7, slightly angled but should be ok.
Should do better next time.

PMT at 445
Some motion but seems fine.
Task, starting visual trials only.

Using normal LED "house lights"

Imaging at surface1, rougly center of the window
Shielding seems fine.
Depth 250 um
Laser 33%
Continuous imaging

Field is not great but see enough neurons to proceed.
Mainly in the center.
Scanners at 2.5V.


Chasing Z drift:
14000 up 2
30000 up 2
33000 up 1
38000 up 1
43000 up 2
48000 up 2
53000 up 2
55000 up 1
58000 up 1
61300 up 1
65500 up 2
71200 up 2
75900 up 1
79000 up 2
81500 up 1
86500 up 2
91500 up 2
96600 up 2
10300 up 2
11100 down 2
11400 up 2
11700 up 3
11900 up 1
12300 up 2
13000 up 2
13400 up 2
13900 up 3
14500 up 2
14800 up 1
15400 up 2
16000 up 2
lost track of the plane. moved around a little bit and came back to where I was before. Maybe subsequent data makes little sense.
17500 up 2
stopped at 174805

Behavior was not great but ok. Visual ~70%, audio at chance. Got worse towards the end.

===============================================================

5/15/18
depth: 170um
angle: ~10 deg
a little bit of light leak from visual stimuli, maybe some movement but hopefully ok
Signal looks good. Many lick tuned neurons. Best one  so far.

Shielding seems fine.
Depth 170 um
Laser 34%
Continuous imaging


Chasing Z drift:
5000 up 1
1000 up 2
1500 up 2
1800 up 1
2200 up 2
2600 up 1
3000 up 2
3700 up 1
4100 up 2
4600 up 2
5500 up 2
6000 up 2
6200 up 1
6400 up 2
6800 up 1
7200 up 2
7600 up 2
8000 up 2
8400 up 2
9200 up 2
9700 up 2
10000 up 2
10500 down 1
10800 down 2
11500 down 2
drift seems to have stopped/slowed down
Fairly certain that we kept the same cells across the session (maybe except for the part where I had to go down again).

pupil is covered with stuff at ~350 trials
behavior was fine. 75% visual, 50% audio. ~400 trials.


===============================================================

5/17/18
angle: 10.5 deg - still slightly angled. Unfolding from posteriorlateral to anteriormedial.
Should be fine though.
depth: 190um
laser power 34%
location is pretty close to where we imaged last time.
signal looks pretty good - many neurons.

Chasing Z drift:
5000 up 1
10000 up 2
some major noise at around 12000-13000. had to open the door to switch on ambient light.
15000 up 2
18000 up 2
21000 up 2
25000 up 1
29000 up 3
32000 up 2
36000 up 2
42000 up 2
49000 up 2
53000 up 2
62000 up 1
65000 down 2
80000 seems like drift is fairly stable now.

Animal has donly only 150 trials though... squinting a lot. Pupil tracking will presumably be not ideal.
Mostly running on vision only to keep the mouse working. Hopefully we'll get enough trials together.
Behavior is pretty weak, ~66% vision, 50% audio. Only 250 performed trials.



===============================================================

5/18/18
angle: 10.5 deg
depth: 175um
laser: 35%

Some movement, light from the stimulus is visible pretty clearly. Can't get it better in place right now. Lots of neurons. SNR is not great - maybe there is some ambient light leaking in somewhere.
Field is good - roughly in the center of the window.

Chasing Z drift:
5000 up 2
10000 up 2
18000 up 2
22000 up 2
29000 up 3
33000 up 2
37000 up 2
42000 up 2
48000 up 2


===========================
08/11/18
Imaging depth is 200um.
Laser at 45%. PMTs at 0.455.
Field looks good. Many active neurons.
MUCH better SNR as in the AI93 mice.

Shielding/movement are good.

Location should match ALM, based on widefield data.
More anteriorlateral to earlier recordings.

No red structural channel in this recording. 
Signal is good enough to be able to use the green channel alone. Using vessel shadows as markers.

20000 up 5
27000 up 5

First 100 trials are vision only, 80% vision afterwards. Performance is pretty bad - close to chance in both conditions.

47000 up 5
56000 up 5

Switched to all audio after 375 performed trials.
Stopped after 440 performed trials.

Performance at chance. Signal is good though and should be stable.


===============================================================
8/24/18
laser: 45%
location: MM. More posteriormedial in the window. Should match based on widefield imaging.
Depth: 180um
Signal looks good, many neurons.
Mild light artefact but should be ok.
Using 1.25% Dextran dye to label vessels.

Switched 15% audio after 100 performed trials. Visual detection is pretty low at ~60%.Switched to all audio after 350 performed tials.
Visual performance still at 60% - not great...


Otherwise good recording, pretty stable, lots of neurons.
Stopped after 420 performed trials.


===============================================================
09/06/18
laser: 48%
location: MM. Roughly same place as last time.
Depth is 185 um. Objective is a bit angled - should adjust before next imaging.

Using 40ul of 1.25% Dextran dye to label vessels.

Shielding is not perfect - see some artifact in the red channel. Green looks ok.

Starting with visual trials only.
Behavior is pretty low in the beginning but improved after ~100 performed trials.
Introducing 10% audio trials after 175 performed trials.
Performance is pretty good up ~performed trial 275. Gets a bit worse after that but recovers at ~trial 300. Should be fine :)

Switched to all audio after 390 performed trials.
Visual detection is ~69%, audio at ~55%.
Stopped after 500 performed trials.


===============================================================
09/14/18
laser: 48%
Location is ALM, based on widefield data. See some lick modulation.
Depth is 195um. 
Signal looks good. Shielding is good.


First 100 performed trials are vision only. 10% audio probability after that.

Switched to all audio after 340 performed trials.
Visual performance at ~66%.


Not very motivated anymore towards the end...
Stopped after 410 performed trials.



Auditory performance at 53%





===============================================================
09/18/18
laser: 60%
Location is ALM, based on widefield data.
Depth is 375um.
Objective angle is 10deg
Signal is darker than in earlier recordings. Not sure why.
Lots of neurons though. Modulated by licking.

Mild light artifact in the red but cant see it in the green.

First 150 performed trials are all vision.
Switched on audio at 10% probability.


Many more neurons over the course of recording and image got notably brighter. Maybe reject some of the eary trials to not undererstimate neural tuning.

Switched to all audio after 375 performed trials. 
Visual detection at ~61%. Audio at 50%.

Stopped after 501 performed trials.



===============================================================
09/22/18
laser: 55%
Location is MM, depth is 370um.
Signal is fine. Very weak signal from the vessel but should be enough.
Many neurons.
Shielding is fine.


First 150 performed trials are all vision.
Switched on audio at 25% probability after that.


===============================================================
09/25/18
laser: 55%
Signal is fine.Many neurons.
Location is MM, depth is 375um. Injected 2x dectran dye.
Vessel signal looks ok. Some movement but should be fine.

First 100 performed trials are vision. Switched to 25% probability audio after that.

Eye is partially blocked - pupil estimate is presumably not very good.

Behavior is decent. 67% vision, 52% audio.

500 performed trials. 
Animal seems stressed towards the end and behavior is not great. Maybe cut the last 50 trials.



===============================================================
09/27/18
laser: 55%
Signal is fine.Many neurons.
Location is ALM, depth is 375um. Injected 1x dectran dye.
Vessel signal looks ok.
Some light leak. Mainly visible in the red channel.

First 100 trials are vision only. Added 25% audio afte rthat.
Pupil signal is not so clear anymore after ~350 performed trials.

Stopped after 475 performed trials.
Vision at 66%, audio at 50%.
Should be a good session.