Triple mouse M
Double glass window, no artificial dura
Behaving in Simon's 2-mode detection task
Visual expert

5/21/18

Objective at 10.8
PMT at 445
Task, starting audio trials only.
Using normal LED "house lights"
Imaging at vessel, rougly center of the window
Shielding seems fine.
Depth 180 um
Laser 32%
Continuous imaging, signal looks good. Much better than other mice so far.
Motion is not that bad, shielding seems fine as well. Should be good.
Scanners at 2.5V.

First file is bad, use the second one.

Chasing Z drift:
6000 up 2
8000 up 1
11000 up 1
12000 up 1
15000 up 1
18000 up 1
23000 up 1
25000 up 1
27000 up 1
29000 up 1
35000 up 1
38000 up 1
70000 up 1
111000 up 2
135000 up 1
141000 up 1
145000 up 1

Drift seems quite slow towards the end. Should be a stable recording throughout.
Some more drift towards the end. Corrected accordingly. Should be fine.

There is a bunch of neurons that are very driven by him cleaning is paws. 
Might be useful for something :)

Towards the end of the session we switched to audio only. after ~400 performed trials we switched to vision only and then back to audio at the very end.
Behavior is not great ~65% audio, 50% vision.
Lots of trials: 500+ performed trials.



===========================
5/21/18

Objective at 10.8
PMT at 445
Task, starting audio trials only.
Using normal LED "house lights"
Laser at 34%
depth is 250um
Imaging roughly in the center of the window. Maybe more posterior to the last time.

Chasing Z drift:
6000 up 2
8000 up 1
12000 up 2
16000 up 1
18000 up 1
22000 up 2
28000 up 2
32000 up 2
40000 up 2
46000 up 1
50000 up 2
54000 up 2
56000 up 2
moved a bit deeper for a moment to check if x and z drift are associated. doesnt seem that way.
seems like neurons are also drifting slightly downwards over time.

60000 up 2
63000 up 2
71000 up 5
73000 up 2

... couldnt take notes for a while. had problems with xy drift and other stuff that I needed to focus on.

101000 up 2
108000 up 2

imaging is not very stable. seems to drift in all three dimensions. damn!
some neurons are fairly constant. have less faith in the upper part of the field.

110000 up 2
113000 up 2
115000 up 3
120000 up 2
125000 up 2
127000 up 1
134000 up 2
138000 up 2
145000 up 2
154000 up 4

159000 down 10
seems like this is closer to the original imaging plane.
This session will be quite drifty overall.


===========================
6/06/18

Objective at 10.8
PMT at 445
Using normal LED "house lights"
Laser at 34%
depth is 200um
Imaging roughly in the anterior center of the window. Should match ALM coordinates.
Shielding seems fine

Had to restart behavior after ~20 trials because camera crashed the paradigm.
Old paradigm is the one that started with visual trials. New paradigm started with audio only.

12000 up 3
20000 up 2
26000 down 3
32000 up 2
35000 up 2
41000 up 3
44000 up 2
52000 up 3
56000 down 3
60000 down 2

didnt take notes on drift for a while - was hard to stay in the same plane.
current position is 154 um

118000 up 2
122000 up 2

tried to change the plane a couple of times but it seems fairly stable.
currently at 150. Many neurons were active over a pretty wide range.

Not sure if the objective actually moved as indicated when trying to change the plane... 
maybe that means the recording was pretty stable :)

Behavior was good ~75% audio, 50% vision.
Eye got occluded at some point so pupile tracking will presumably not very good.

Hopefully good session overall.



===========================
06/08/18

Objective at 10.8
PMT at 445
Using normal LED "house lights"
Laser at 32%
depth is 200um
Imaging roughly in the anterior center of the window. Should match ALM coordinates.
Shielding seems fine
Good field - signal looks fine
Quite a bit movement

10000 up 2
28000 up 2

lots of neurons in the field - really hard to see if the field is changing or not...

39000 up 2
85000 up 2
100000 up 2

Pupil is filled at ~trial300. Tracking is presumably no good.
Switched to all visual at trial 400 to get some more novice trials.

130000 down 2
150000 up 2

Should be a good session. Very little drift - performance at ~76% for audio, 50% vision.
Stopped after 500 performed trials.



===========================
06/12/18

Objective at 10.8
PMT at 445
Laser at 34%
depth is 400um
Imaging roughly in the anterior center of the window. Should match ALM coordinates.
Close to the field that we imaged the last time I think.
Shielding is no good. Won't use visual stimuli in this session.
Good field - signal looks fine. Darker than usual but should be ok. Lots of neurons. 
Clearly clustered. 
Which different events recruiting other pattern of neurons - hopefully thats not Z-motion.
Looks really interesting. Seems like there is much more tuning as in superficial layers.

18000 up 10
22000 up 2
25000 up 2
29000 up 2
34000 up 2
37000 up 2
40000 up 2
47000 up 2
50000 down 3
52000 up 4
56000 up 2
63000 up 2
68000 up 4
72000 up 2
76000 up 2

Area seems REALLY active when the mouse is licking its paw.

Had to move back down a bit at around trial 300. Seems like I overshot or drift changed direction at some point. Might be a bit drifty again.

Behavior is decent. ~72%, 450 performed trials.


===========================
06/15/18

Objective at 10.8
PMT at 445
Laser at 35%
depth is 375um
Position is posterior/medial. Should match MM coordinates.
Good field. Signal seems fine.
Movement seems ok.
Not using the cone this time, so auditory only.

11000 up 2
15000 up 1
25000 up 2
38000 up 2
55000 up 2
63000 up 3
66000 up 1
74000 up 2
105000 up 2

Should be a nice session. Animal behavior is 79% audio, no vision.
400 performed trials.



===========================
06/22/18

Objective at 10.8
PMT at 445
Laser at 35%
depth is 415um
Position is anterior/lateral. Should match ALM coordinates.
Good field. Signal is not the best but should be ok.
Movement seems ok. Shielding is good.

11000 up 2
18000 up 2
26000 up 2

Didnt take notes of drift for a while. POsition is 395 at 55000.
390 at 100000. Seems pretty stable.

Did mostly visual trials between performed trials 350-400.
Good session overall. Audio performance ~77%, vision at chance. 400 performed trials.


===========================
06/27/18

Objective at 10.8
PMT at 445
Laser at 35%
depth is 395um
Position is posterior/medial. Should match MM coordinates.
Good field. Movement seems ok. Shielding is good.

MScan crashed when trying to stream data. Had to restart behavior and imaging system.
Make sure to use correct behavioral data.

10000 up 3
15000 up 2

Some premature licking in some trials

20000 up 2
30000 up 2

Plane is mostly stable. Should be a good recording.
Animal performance is decent ~75% audio, %50 vision
Switched to vision only after 410 performed trials.
At ~440 performed trials, the behavior system lost connection to one of the webcams. 
Stopped recording -> Check for last trial with both cameras and trim session accordingly.



===========================
07/19/18

Objective at 7.1
Depth is 215um
Shielding is good, will do audio + visual trials.
Did two stacks from 195 to 235um. First file is with 10frame average, the second with 20frame average. Think 20 will come out better.

15000 xy adjustment
25000 up 5, xy adjustment

Started presenting visual trials at 25% after 100 performed trials.

32000 up 4

Behavior is not very good. Set auditory probability to 90% and will present more visual trials toward the end.

90000 up 4

Switched to all vision after 350 performed trials.

110000 up 4

Pretty weak performance. Audio detection ~60%, vision at chance.

Stopped after 436 performed trials.




===========================
08/24/18
Depth is 180um
Location is MM - slightly more anterior this time.
Signal looks good. Laser at 40%.
Shielding is good.
Injected 1.25% of dextran dye. Lost some volume though so it was more ~0.7%.
Still looks usable.

Quite a bit of spontaneous licking before spouts are moving in.
Started vision after 100 auditory trials at 15%.
Switched to all vision after 375 performed trials.
Stopped after 470 performed trials.
Performance is 65% audio, 53% vision