Triple mouse M
Double glass window, no artificial dura
Behaving in Simon's 2-mode detection task
Audiory expert

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7/27/18
Imaging depth is 220um.
Location should match ALM.
SNR is not great but should be good enough.
Many neurons.
Red channel has tdTomato tag. Seems to show axons/dendrites instead of cell bodies but good for structure.

Laser power is 44%.
Shielding is ok, presenting both audio and visual stimuli. 

Drift seems fine.

42000  up 10 
90000  up 5
116000 up 5

Behavior is good ~75% in auditory. Chance in vision.
Ran at 0.8/0.2 for audio and vision after ~130 performed trials. All audio before that.
Switched to all vision after 410 performed trials.
Stopped after 475 performed trials.

Should be a good recording. Good data/behavior. Right place.
Also seems to have less motion than the other mice.


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7/28/18
Imaging depth is 180um.
Location should match MM.
Anteriormedial from large blood vessel.
Signal is not as good usual and the objective is angled. Medial part is higher by ~60um. Will try to adjust and hopefuly do better next time.

Laserpower is 44%. Some neurons, low SNR.
First file is stack of 40um in 2um steps. Starting at 160um downwards.

25000 up 5
80000 up 5

Animal seems pretty disengaged after 320 performed trials.
Switched to all vision to get some more visual trials in.
Performance is good. 70% audio, 54% vision.
Stopped after 415 performed trials.
Drift seemed fine - should be stable recording although brightness was increasing over time.

Not 100% sure about the location because the vessel images were not ideal. Should be at the mark from the widefield but there is a small chance it ended up more posterior. Check later!

Changed objective angle from 7.2 to 8.5 after the session.