Triple mouse M
Double glass window, no artificial dura
Behaving in Simon's 2-mode detection task
Auditory expert. Window centered over PPC.

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8/03/18
Imaging depth is 180um.
Location should match RL.
SNR is pretty bad. There is massive bleed through from the red channel into the green. Expression seems much brighter than Gcamp modulation. Can't really see much change by eye...
Recording anyways just to see whether this might be useful.

Should use much less virus the next time!
Pretty good otherwise. Very little movement with licking.

Started presenting visual trials with 20% probability after 65 performed trials.

So annoying... everything else is really nice, behavior is good, virtually no drift / movement. Just barely any activity in the green channel.

Something seems wrong with the animals eye: I can't see the pupil although the laser is clearly running. Usually the pupil should be illuminated. Maybe, this doesn't happen with the PPC window - check if the pupil can still be extracted...


70000 up 5

Switched to all vision after 345 performed trials. Animal got pretty disengaged somewhere in the middle of the session and behavior is not as good. Still at 75% audio detection though.


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8/03/18
Imaging depth is 180um.
Laser at 48%.
Location should match V1 - mostly posterior to RL.
Very few modulated neurons.
Very little motion, shielding is good.
Pupil seems dark instead of bright.

25000 up 5

Bpod matlab crashed around frame 43000. Restarted.

90000 up 5

Switched to all vision after 230 performed trials in new session.
Auditory detection ~73%/ Audio probability at 80%.
Stopped after 310 performed trials.