{ "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "FrameKey-0-0-0": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 85.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "815", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "638cb030-cc06-4c11-b487-5a5f119d2bb2", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_000.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 0, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465028920.87", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:28 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "0", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 21548, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "731", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "1", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:28.920871", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-1": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 87.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "1315", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "5366c108-a509-4ad5-8e8e-34954ae74124", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_001.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 1, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465028970.85", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:28 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "1", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 21597.98, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "1232", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "2", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:28.970846", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-2": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 89.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "1816", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "4ce6f1dc-914b-4e18-bb5d-4e6d0e250bab", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_002.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 2, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029020.86", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "2", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 21647.989999999998, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "1732", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "3", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.020865", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-3": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 91.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "2316", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "b76453d7-79e3-4042-80fa-bf91cf7a0c43", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_003.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 3, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029070.86", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "3", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 21697.989999999998, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "2232", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "4", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.070857", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-4": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 93.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "2816", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "bfd854e3-09d7-4694-bec6-1f7f6c4f4be8", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_004.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 4, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029120.86", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "4", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 21747.989999999998, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "2732", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "5", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.120859", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-5": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 95.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "3316", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "48677bc4-71bd-4b3d-a95b-e147aaad9ea7", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_005.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 5, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029170.86", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "5", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 21797.989999999998, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "3232", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "6", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.170860", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-6": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 97.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "3816", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "979f8bd9-5a1d-46ee-a037-15aa63eb3790", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_006.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 6, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029220.83", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "6", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 21847.96, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "3733", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "7", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.220829", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-7": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 99.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "4316", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "c48e1d11-b45e-4665-98ae-940e12f4dc11", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_007.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 7, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029270.86", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "7", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 21897.989999999998, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "4233", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "8", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.270856", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-8": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 101.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "4816", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "14d11f1d-80fe-49e7-b295-014a7a339f79", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_008.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 8, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029320.86", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "8", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 21947.989999999998, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "4733", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "9", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.320856", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-9": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 103.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "5317", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "b72a0c9a-80ef-488f-8556-de8b220d3f9b", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_009.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 9, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029370.87", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "9", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 21998, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "5233", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "10", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.370867", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-10": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 105.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "5817", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "3af3ef18-8d81-4f9d-bfd7-a17b1bd255ba", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_010.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 10, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029420.85", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "10", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22047.98, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "5733", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "11", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.420852", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-11": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 107.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "6317", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "64740439-6987-46d3-9289-603f6171382a", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_011.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 11, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029470.85", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "11", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22097.98, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "6233", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "12", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.470849", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-12": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 109.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "6817", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "073667c0-de0e-4813-86e2-bad6055019f9", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_012.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 12, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029520.84", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "12", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22147.97, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "6734", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "13", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.520844", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-13": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 111.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "7317", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "6280c8b8-f1d0-46bf-9d29-ef1e8c19d666", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_013.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 13, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029570.85", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "13", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22197.98, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "7234", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "14", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.570847", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-14": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 113.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "7818", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "542f59a6-c789-4920-b384-55722b2ee58d", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_014.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 14, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029620.84", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "14", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22247.98, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "7734", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "15", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.620845", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-15": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 115.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "8318", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "77b81de0-2550-4fac-93da-125e7a461f95", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_015.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 15, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029670.84", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "15", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22297.97, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "8234", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "16", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.670844", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-16": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 117.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "8818", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "88dc8e73-4c88-44b5-a7ff-1ce4d7c213e2", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_016.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 16, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029720.81", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "16", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22347.94, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "8734", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "17", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.720815", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-17": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 119.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "9318", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "229aa0e3-a913-4d5e-941d-fe747c62638d", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_017.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 17, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029770.83", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "17", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22397.96, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "9235", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "18", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.770834", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-18": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 121.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "9818", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "3ea28c58-575a-4bbb-992d-f853b6685d3b", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_018.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 18, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029820.84", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "18", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22447.97, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "9735", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "19", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.820837", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-19": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 123.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "10319", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "56b179a7-aeb8-4dc2-8bcd-6034776579a1", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_019.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 19, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029870.84", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "19", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22497.97, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "10235", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "20", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.870838", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-20": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 125.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "10819", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "3037d977-d35f-42b9-9385-3a68cf7a2f29", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_020.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 20, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029920.84", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "20", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22547.97, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "10735", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "21", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.920837", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-21": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 127.456, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "11319", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "44bf2b83-edd8-41b0-85b6-542f0e6dc1c7", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_021.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 21, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465029970.84", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:29 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "21", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22597.97, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "11235", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "22", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:29.970836", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-22": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 129.45600000000002, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "11819", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "e7d7392b-9636-4ff3-919d-d09e9560178b", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_022.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 22, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465030020.83", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:30 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "22", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22647.96, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "11736", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "23", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:30.020830", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-23": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 131.45600000000002, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "12319", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "8d5b8e95-e26c-4ef5-b510-5396b6524669", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_023.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 23, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465030070.83", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:30 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "23", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22697.96, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "12236", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "24", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:30.070834", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-24": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 133.45600000000002, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "12819", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "5827fc35-0869-4e9b-8176-7e0c6869217f", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_024.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 24, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465030120.83", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:30 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "24", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22747.96, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "12736", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "25", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:30.120826", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-25": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 135.45600000000002, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "13320", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "f4fc8557-830c-4780-ad9f-9cb5303f92e2", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_025.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 25, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465030170.83", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:30 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "25", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22797.96, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "13236", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "26", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:30.170832", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-26": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 137.45600000000002, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "13820", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "3608cad9-2925-478e-a080-4623b8ab0dfe", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_026.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 26, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465030220.83", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:30 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "26", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22847.96, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "13736", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "27", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:30.220828", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-27": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 139.45600000000002, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "14320", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "dcda10d2-23a6-42b4-a99b-fa6ec76a35c6", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_027.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 27, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465030270.81", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:30 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "27", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22897.94, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "14236", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "28", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:30.270814", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-28": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 141.45600000000002, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "14820", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "9f8f5466-5676-4678-bb56-3e5469927fea", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_028.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 28, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465030320.83", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:30 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "28", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22947.96, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "14737", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "29", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:30.320826", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-29": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 143.45600000000002, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "15321", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "0f037c5c-8743-4624-8ca5-1fe1bdf854a4", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_029.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 29, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465030370.82", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:30 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "29", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 22997.96, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "15237", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "30", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:30.370825", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" }, "FrameKey-0-0-30": { "Objective-Name": "Objective", "COM6-Fast USB to Serial": "Disable", "Condenser-Name": "Condenser", "Core-Initialize": "1", "Prime BSI-TransposeCorrection": "0", "405-DA Device": "405 Shutter", "COM7-AnswerTimeout": "500.0000", "555-Name": "DA Shutter", "COM7-Handshaking": "Off", "528-State": "0", "405 AO-Sequencing": "Use hub setting", "405 Shutter-MaxVolts": "5.0000", "640-Name": "DA Shutter", "520 AO-Sequencing": "Use hub setting", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "Prime BSI-Gain": "1-HDR", "ROI": "0-0-2048-2048", "Prime BSI-PP 2 THRESHOLD": "120", "FilterTurretOLYMPUS-Label": "Position-6", "Prime BSI-Port": "CMOS", "Prime BSI-ClearCycles": "2", "XYStage-StepSizeX_um": "1.0000", "555 Shutter-MaxVolts": "5.0000", "Objective-Safe Position": "100.0000", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "OlympusHub-Model": "IX2", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 145.45600000000002, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "NIMultiAnalogOutHub-1-Sequence": "On", "Prime BSI-Exposure": "50.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "Exposure-ms": 50, "NIMultiAnalogOutHub-1-TriggerInputPort": "/dev1/PFI0", "405 AO-MaxVolts": "5.0000", "PVCAM-TimeStamp": "15821", "520 Shutter-MinVolts": "0.0000", "445 AO-Sequencing": "Use hub setting", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "640 AO-Sequencing": "Use hub setting", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "COM7-Parity": "None", "555 Shutter-MinVolts": "0.0000", "Prime BSI-Description": "PVCAM API device adapter", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "Prime BSI-CircularBufferAutoSize": "ON", "AxisPositions": null, "Objective-Label": "20x UCPlanFLN", "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "LudlController-Name": "LudlController", "470 AO-Sequencing": "Use hub setting", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "ManualFocus-NearLimit": "30000.0000", "528 Shutter-Voltage": "5.0000", "640 AO-Voltage": "0.0000", "XLIGHT Spin motor-State": "1", "528 AO-Sequencing": "Use hub setting", "445 Shutter-Voltage": "5.0000", "PI AO-Voltage": "5.2480", "Prime BSI-ShutterOpenDelay": "0", "XYStage-Port": "COM4", "COM7-StopBits": "2", "XYStage-MaxSpeed": "100", "555 Shutter-Sequencing": "Use hub setting", "445 Shutter-Sequencing": "Use hub setting", "555-State": "1", "520 AO-Voltage": "0.0000", "Prime BSI-Offset": "100", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7400", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "Prime BSI-ShutterMode": "Pre-Exposure", "COM3-AnswerTimeout": "500.0000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "COM7-Fast USB to Serial": "Disable", "445-Description": "DA device that is used as a shutter", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "528 AO-Voltage": "0.0000", "445-Name": "DA Shutter", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "405 Shutter-Sequencing": "Use hub setting", "COM3-Handshaking": "Off", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640 Shutter-Sequencing": "Use hub setting", "640-DA Device": "640 Shutter", "520-State": "0", "UUID": "d3bffd7f-ee7f-4b39-b23b-3ad94a1a2258", "Core-Camera": "Prime BSI", "Core-SLM": "", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "7", "DA Z Stage-Stage High Voltage": "10.0000", "470 Shutter-Sequencing": "Use hub setting", "405 Shutter-Voltage": "5.0000", "LightPath-Description": "Light Path Prism", "Core-ChannelGroup": "Channels1", "PositionIndex": 6, "WaitInterval": null, "OlympusHub-Description": "Olympus Hub", "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "PixelSizeAffine": "1.0;0.0;0.0;0.0;1.0;0.0", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "520 AO-MinVolts": "0.0000", "470 AO-MaxVolts": "5.0000", "Prime BSI-CircularBufferFrameRecovery": "OFF", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.341, "Prime BSI-PVCAM Adapter Version": "1.3.60", "528 AO-MinVolts": "0.0000", "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 31, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8468.55], "XYStage": [ 57439, 7847 ] }, "GridColumnIndex": 0, "Label": "Pos0", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473.44], "XYStage": [ 59501, 4054 ] }, "GridColumnIndex": 0, "Label": "Pos1", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477], "XYStage": [ 57104, 3501 ] }, "GridColumnIndex": 0, "Label": "Pos2", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8473], "XYStage": [ 56557, 3991 ] }, "GridColumnIndex": 0, "Label": "Pos3", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8483.02], "XYStage": [ 65858, 3727 ] }, "GridColumnIndex": 0, "Label": "Pos4", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.85], "XYStage": [ 68071, -2278 ] }, "GridColumnIndex": 0, "Label": "Pos5", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.36], "XYStage": [ 65572, -545 ] }, "GridColumnIndex": 0, "Label": "Pos6", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.18], "XYStage": [ 64957, 26 ] }, "GridColumnIndex": 0, "Label": "Pos7", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8464.25], "XYStage": [ 72726, 11327 ] }, "GridColumnIndex": 0, "Label": "Pos8", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8466.82], "XYStage": [ 73849, 8720 ] }, "GridColumnIndex": 0, "Label": "Pos9", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.69], "XYStage": [ 71660, 7128 ] }, "GridColumnIndex": 0, "Label": "Pos10", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8477.02], "XYStage": [ 71158, 7695 ] }, "GridColumnIndex": 0, "Label": "Pos11", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8497.64], "XYStage": [ 57747, -17421 ] }, "GridColumnIndex": 0, "Label": "Pos12", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8507.83], "XYStage": [ 60095, -20377 ] }, "GridColumnIndex": 0, "Label": "Pos13", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8508.28], "XYStage": [ 57295, -21854 ] }, "GridColumnIndex": 0, "Label": "Pos14", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8506.13], "XYStage": [ 56714, -21076 ] }, "GridColumnIndex": 0, "Label": "Pos15", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8520.28], "XYStage": [ 62914, -24132 ] }, "GridColumnIndex": 0, "Label": "Pos16", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8527.5], "XYStage": [ 66132, -26376 ] }, "GridColumnIndex": 0, "Label": "Pos17", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.97], "XYStage": [ 62452, -28519 ] }, "GridColumnIndex": 0, "Label": "Pos18", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8526.57], "XYStage": [ 61893, -27876 ] }, "GridColumnIndex": 0, "Label": "Pos19", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8500.28], "XYStage": [ 69848, -15283 ] }, "GridColumnIndex": 0, "Label": "Pos20", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.71], "XYStage": [ 72537, -17840 ] }, "GridColumnIndex": 0, "Label": "Pos21", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8515.2], "XYStage": [ 69348, -19736 ] }, "GridColumnIndex": 0, "Label": "Pos22", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8513.42], "XYStage": [ 68753, -18903 ] }, "GridColumnIndex": 0, "Label": "Pos23", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8460.23], "XYStage": [ 578, 7420 ] }, "GridColumnIndex": 0, "Label": "Pos24", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.78], "XYStage": [ 3506, 2853 ] }, "GridColumnIndex": 0, "Label": "Pos25", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.88], "XYStage": [ 143, 2997 ] }, "GridColumnIndex": 0, "Label": "Pos26", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8465.48], "XYStage": [ -208, 3790 ] }, "GridColumnIndex": 0, "Label": "Pos27", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.23], "XYStage": [ 9333, 1393 ] }, "GridColumnIndex": 0, "Label": "Pos28", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8467.02], "XYStage": [ 11640, -1523 ] }, "GridColumnIndex": 0, "Label": "Pos29", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8472.55], "XYStage": [ 8535, -2822 ] }, "GridColumnIndex": 0, "Label": "Pos30", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8471.5], "XYStage": [ 8068, -2378 ] }, "GridColumnIndex": 0, "Label": "Pos31", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8443.54], "XYStage": [ 15238, 9559 ] }, "GridColumnIndex": 0, "Label": "Pos32", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8448.83], "XYStage": [ 17798, 6980 ] }, "GridColumnIndex": 0, "Label": "Pos33", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8455.29], "XYStage": [ 14742, 5073 ] }, "GridColumnIndex": 0, "Label": "Pos34", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.43], "XYStage": [ 13930, 5933 ] }, "GridColumnIndex": 0, "Label": "Pos35", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.28], "XYStage": [ 1123, -18619 ] }, "GridColumnIndex": 0, "Label": "Pos36", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8491.96], "XYStage": [ 3444, -21358 ] }, "GridColumnIndex": 0, "Label": "Pos37", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.98], "XYStage": [ 274, -22750 ] }, "GridColumnIndex": 0, "Label": "Pos38", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8485.75], "XYStage": [ -177, -22215 ] }, "GridColumnIndex": 0, "Label": "Pos39", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8503.3], "XYStage": [ 8084, -24151 ] }, "GridColumnIndex": 0, "Label": "Pos40", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8501.17], "XYStage": [ 10017, -27515 ] }, "GridColumnIndex": 0, "Label": "Pos41", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.36], "XYStage": [ 7145, -27627 ] }, "GridColumnIndex": 0, "Label": "Pos42", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8499.75], "XYStage": [ 6558, -27554 ] }, "GridColumnIndex": 0, "Label": "Pos43", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.42], "XYStage": [ 14812, -15439 ] }, "GridColumnIndex": 0, "Label": "Pos44", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8480.34], "XYStage": [ 17365, -17541 ] }, "GridColumnIndex": 0, "Label": "Pos45", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.6], "XYStage": [ 14178, -19353 ] }, "GridColumnIndex": 0, "Label": "Pos46", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.83], "XYStage": [ 13677, -19089 ] }, "GridColumnIndex": 0, "Label": "Pos47", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8474.69], "XYStage": [ -13565, -14111 ] }, "GridColumnIndex": 0, "Label": "Pos48", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8479.84], "XYStage": [ -16115, -11449 ] }, "GridColumnIndex": 0, "Label": "Pos49", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8487.73], "XYStage": [ -13119, -10062 ] }, "GridColumnIndex": 0, "Label": "Pos50", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8488.83], "XYStage": [ -12506, -10526 ] }, "GridColumnIndex": 0, "Label": "Pos51", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8458.56], "XYStage": [ -19358, -6386 ] }, "GridColumnIndex": 0, "Label": "Pos52", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430.5], "XYStage": [ -21803, -2983 ] }, "GridColumnIndex": 0, "Label": "Pos53", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8444.74], "XYStage": [ -18743, -2305 ] }, "GridColumnIndex": 0, "Label": "Pos54", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8453.67], "XYStage": [ -18159, -2813 ] }, "GridColumnIndex": 0, "Label": "Pos55", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8416.98], "XYStage": [ -26819, -14543 ] }, "GridColumnIndex": 0, "Label": "Pos56", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8401.98], "XYStage": [ -29265, -11779 ] }, "GridColumnIndex": 0, "Label": "Pos57", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8419.28], "XYStage": [ -26044, -10419 ] }, "GridColumnIndex": 0, "Label": "Pos58", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8422.88], "XYStage": [ -25690, -11096 ] }, "GridColumnIndex": 0, "Label": "Pos59", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8456.14], "XYStage": [ -13378, 11213 ] }, "GridColumnIndex": 0, "Label": "Pos60", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8445.57], "XYStage": [ -15745, 13113 ] }, "GridColumnIndex": 0, "Label": "Pos61", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8454.16], "XYStage": [ -12697, 15262 ] }, "GridColumnIndex": 0, "Label": "Pos62", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8457.13], "XYStage": [ -12162, 14712 ] }, "GridColumnIndex": 0, "Label": "Pos63", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8430], "XYStage": [ -19567, 19181 ] }, "GridColumnIndex": 0, "Label": "Pos64", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8413.14], "XYStage": [ -22085, 21127 ] }, "GridColumnIndex": 0, "Label": "Pos65", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8426.3], "XYStage": [ -19049, 23285 ] }, "GridColumnIndex": 0, "Label": "Pos66", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8429.88], "XYStage": [ -18631, 22671 ] }, "GridColumnIndex": 0, "Label": "Pos67", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8409.88], "XYStage": [ -24939, 10791 ] }, "GridColumnIndex": 0, "Label": "Pos68", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8396.23], "XYStage": [ -27376, 12931 ] }, "GridColumnIndex": 0, "Label": "Pos69", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8410.82], "XYStage": [ -24547, 14933 ] }, "GridColumnIndex": 0, "Label": "Pos70", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8415.61], "XYStage": [ -23851, 14260 ] }, "GridColumnIndex": 0, "Label": "Pos71", "GridRowIndex": 0 } ], "Interval_ms": 0, "UUID": "af78e2c5-2d0f-409d-9e53-fe4f794653b3", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2023-08-04 11:50:28 -0400", "Date": "2023-08-04", "z-step_um": 2, "MetadataVersion": 10, "PositionIndex": 6, "SlicesFirst": true, "ChContrastMin": [0], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.24 20221227", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": ["P-TexasRed"], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "focus_1", "PixelSize_um": 0.341, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 1, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [-65281], "TimeFirst": false, "ChContrastMax": [65536], "Positions": 72, "Directory": "F:\\07272023_RDG_HZ Cerebellum Center #4-2, #5-1, #6-1_Genes" }, "Source": "Prime BSI", "Prime BSI-ScanLineDelay": "0", "Prime BSI-PVCAM Version": "3.10.0", "XLIGHT Dichroic-Label": "Di02-R594", "COM4-StopBits": "1", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "COM4-DataBits": "8", "COM3-DataBits": "8", "445 AO-MaxVolts": "5.0000", "DA Z Stage-Stage High Position(um)": "220.0000", "Condenser-ClosedPosition": "", "TransmittedLamp-Description": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "FilterTurretOLYMPUS-Name": "FilterCube", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "10.0000", "Prime BSI-DiskStreamingPath": "", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405-State": "0", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "470 AO-Voltage": "0.0000", "OlympusHub-Port": "COM3", "XYStage-TransposeMirrorY": "0", "XPositionUm": 65572, "Shutter1-Description": "Shutter Number 1", "XYStage-TransposeMirrorX": "0", "Prime BSI-DiskStreamingEnabled": "No", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "470-DA Device": "470 Shutter", "LudlShutter-LudlDeviceNumberShutter": "1", "FileName": "img_000000000_P-TexasRed_030.tif", "XYStage-Acceleration": "100", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "Core-Galvo": "", "Prime BSI-ScanLineTime": "11200000.0000", "Position": "Default", "LightPath-Name": "LightPath", "CameraChannelIndex": 0, "SliceIndex": 30, "ZPositionUm": 30, "528-DA Device": "528 Shutter", "Core-Focus": "DA Z Stage", "Channel": "P-TexasRed", "Prime BSI-ScanWidth": "0", "XLIGHT Excitation Wheel-Label": "Quad", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "43", "FrameIndex": 0, "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "COM6-BaudRate": "9600", "640 Shutter-MaxVolts": "5.0000", "555 AO-Voltage": "1.0000", "COM6-Description": "Serial port driver (boost:asio)", "TransmittedLamp-State": "1", "555 AO-MinVolts": "0.0000", "DA Z Stage-DA Device": "PI AO", "555 AO-Sequencing": "Use hub setting", "TimeStampMsec": "744465030420.82", "Camera": "Prime BSI", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Voltage": "0.0000", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-6", "COM4-Parity": "None", "Core-ImageProcessor": "", "XLIGHT Hub-Name": "XLIGHT Hub", "Prime BSI-Timing-ExposureTimeNs": "100005000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "XYStage-StepSizeY_um": "1.0000", "Prime BSI-StartTime-ms": "744465028838.76", "Prime BSI-ShutterCloseDelay": "0", "Prime BSI-PP 6 ENABLED": "No", "520 Shutter-Voltage": "5.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "Core-AutoShutter": "0", "COM7-DelayBetweenCharsMs": "0.0000", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "Prime BSI-Color": "OFF", "XLIGHT Dichroic-State": "2", "TransmittedLamp-ClosedPosition": "", "Prime BSI-ScanDirectionReset": "Yes", "Prime BSI-DiskStreamingCoreSkipRatio": "1:100", "NextFrame": null, "Core-XYStage": "XYStage", "ManualFocus-Position": "8493.0000", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "Prime BSI-PP 3 THRESHOLD": "85", "Prime BSI-ScanDirection": "Down", "Shutter1-State": "0", "COM6-StopBits": "1", "Time": "2023-08-04 11:50:30 -0400", "COM7-Verbose": "1", "405 AO-Voltage": "0.0000", "PI AO-MinVolts": "0.0000", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "ImageNumber": "30", "Core-AutoFocus": "", "640-State": "0", "Objective-State": "5", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "640 AO-MinVolts": "0.0000", "520-Description": "DA device that is used as a shutter", "555 Shutter-Voltage": "5.0000", "470 Shutter-Voltage": "5.0000", "Prime BSI-PreampOffLimit": "10000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "COM6-Verbose": "0", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "NIMultiAnalogOutHub-1-Device": "dev1", "Prime BSI-TriggerMode": "Internal Trigger", "Condenser-State": "5", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "Prime BSI-ScanMode": "Auto", "445 AO-MinVolts": "0.0000", "Core-Shutter": "555", "COM7-BaudRate": "9600", "XLIGHT Hub-Port": "COM6", "Core-TimeoutMs": "5000", "Prime BSI-ActualInterval-ms": "702.9200", "520 AO-MaxVolts": "5.0000", "ElapsedTime-ms": 23047.95, "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-Voltage": "5.0000", "Prime BSI-SerialNumber": "A23E204001", "555-Description": "DA device that is used as a shutter", "445-State": "0", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "528 Shutter-Sequencing": "Use hub setting", "Prime BSI-PP 1 THRESHOLD": "115", "Frame": 0, "520-Name": "DA Shutter", "Width": 2048, "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "COM3-StopBits": "2", "PositionName": "Pos6", "XLIGHT Spin motor-Label": "On (spinning)", "PVCAM-ReadoutTime": "0", "528 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "520 Shutter-Sequencing": "Use hub setting", "COM3-Verbose": "1", "COM3-DTR": "Disable", "Objective-Use Safe Position": "1", "Prime BSI-PP 5 ENABLED": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "COM6-DTR": "Disable", "PVCAM-CameraHandle": "0", "COM3-Name": "COM3", "PVCAM-TimeStampBOF": "15737", "LudlController-Reset": "Operate", "OlympusHub-Control": "Manual + Computer", "COM7-DTR": "Disable", "XLIGHT Hub-Description": "XLIGHT hub", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "XLIGHT Hub-1-Description": "XLIGHT hub", "Prime BSI-Y-dimension": "2048", "PVCAM-FrameNr": "31", "Prime BSI-Trigger Timeout (secs)": "10", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "XYStage-SCurve": "100", "PixelType": "GRAY16", "LudlController-Port": "COM7", "Prime BSI-PP 2 ENABLED": "Yes", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "Prime BSI-PP 7 Reset": "No", "COM4-AnswerTimeout": "500.0000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "YPositionUm": -545, "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "ManualFocus-FocusWheel": "Off", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "LudlShutter-State": "0", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "DA Z Stage-Position": "115.4560", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "COM4-DTR": "Disable", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "5.0000", "445 Shutter-MinVolts": "0.0000", "COM3-BaudRate": "19200", "FilterTurretOLYMPUS-ClosedPosition": "", "TimeReceivedByCore": "2023-08-04 11:50:30.420824", "NIMultiAnalogOutHub-1-MaxSequenceLength": "1024", "PI AO-Sequencing": "Use hub setting", "Prime BSI-FirmwareVersion": "23.41", "COM4-Fast USB to Serial": "Disable", "NIMultiAnalogOutHub-1-SampleRateHz": "10000.0000" } }