### the original raw data (size selection):
/seq/Illumina_runs/NextSeqData/NextSeqOutput/211119_NB501555_1009_AHCN7MAFX3/Data/Intensities/BaseCalls/309509
	(126,408,712)
In node bamdev1: enter the folder: /seq/Illumina_runs/NextSeqData/NextSeqOutput/211119_NB501555_1009_AHCN7MAFX3/Data/Intensities/BaseCalls/309509
	scp *.* zwang@wigclust20:/mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-06_SC-seq-data_Elaine_NextSeq/SizeSelection/.
	### wait the copying finished...

processed data: /mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-06_SC-seq-data_Elaine_NextSeq/SizeSelection/

	
### deconvolute the barcodes:
/mnt/wigclust15/data/safe/leey/ArXiv/ToWhom/zihua/code/sfbc.differnet.barcodes.length/trimBarcodeFragments -nThreads=8 -inputFiles=/mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-06_SC-seq-data_Elaine_NextSeq/SizeSelection/CE8_Nlalll-Tail_SS_S1_R1_001.fastq.gz,/mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-06_SC-seq-data_Elaine_NextSeq/SizeSelection/CE8_Nlalll-Tail_SS_S1_R2_001.fastq.gz "1-G1-50pg-ss:GTCAGCT;TCTCACCT;GGTATTCGT;AATTGATGCT" "2-HU90-50pg-ss:CACTCGT;CTAGACGT;ACGACAGCT;TGGCATACGT" "3-G1-1ng-ss:TCAAGCT;ATTGACGT;CAGATTCCT;TGTCGATAGT" "4-HU90-1ng-ss:CGTTCGT;GCATAGGT;AAGGATGCT;GTCGTAAGCT" "5-G1-50pg-ss:AGGACCT;TAACAGCT;TGAGTTGGT;CAGTGAACCT" "6-HU90-50pg-ss:GAGTGCT;GTTATCCT;ACAGCAGCT;TGAACATGCT" "7-G1-1ng-ss:AGGAGGT;AGTCTGGT;TCTGTACCT;CGACATACGT" "8-HU90-1ng-ss:CTATGGT;GACTTCCT;GGCACTGCT;GACTGTTCCT"

UNCLASSIFIED_pairCnt:	17668643
MISMATCH_pairCnt:	145707
1-G1-50pg-ss_pairCnt:	12941546
2-HU90-50pg-ss_pairCnt:	13059245
3-G1-1ng-ss_pairCnt:	13958002
4-HU90-1ng-ss_pairCnt:	14208685
5-G1-50pg-ss_pairCnt:	14176132
6-HU90-50pg-ss_pairCnt:	14335600
7-G1-1ng-ss_pairCnt:	12530934
8-HU90-1ng-ss_pairCnt:	13384218



### the original raw data (double purification):
/seq/Illumina_runs/NextSeqData/NextSeqOutput/211119_NB551387_0664_AHCN2YAFX3/Data/Intensities/BaseCalls/309508
	(131,641,824)	
In node bamdev1: enter the folder: /seq/Illumina_runs/NextSeqData/NextSeqOutput/211119_NB551387_0664_AHCN2YAFX3/Data/Intensities/BaseCalls/309508
	scp *.* zwang@wigclust20:/mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-06_SC-seq-data_Elaine_NextSeq/DoublePurification/.
	### wait the copying finished...

/mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-06_SC-seq-data_Elaine_NextSeq/DoublePurification/

	
### deconvolute the barcodes:
“.1” and “.5” are G1 with 50pg input.
“.2” and “.6” are HU90 with 50pg input.
“.3” and “.7” are G1 with 1ng input.
“.4” and “.8” are HU90 with 1ng input.


/mnt/wigclust15/data/safe/leey/ArXiv/ToWhom/zihua/code/sfbc.differnet.barcodes.length/trimBarcodeFragments -nThreads=8 -inputFiles=/mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-06_SC-seq-data_Elaine_NextSeq/DoublePurification/CE8_Nlalll_Tail_DP_S1_R1_001.fastq.gz,/mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-06_SC-seq-data_Elaine_NextSeq/DoublePurification/CE8_Nlalll_Tail_DP_S1_R2_001.fastq.gz "1-G1-50pg-dp:GTCAGCT;TCTCACCT;GGTATTCGT;AATTGATGCT" "2-HU90-50pg-dp:CACTCGT;CTAGACGT;ACGACAGCT;TGGCATACGT" "3-G1-1ng-dp:TCAAGCT;ATTGACGT;CAGATTCCT;TGTCGATAGT" "4-HU90-1ng-dp:CGTTCGT;GCATAGGT;AAGGATGCT;GTCGTAAGCT" "5-G1-50pg-dp:AGGACCT;TAACAGCT;TGAGTTGGT;CAGTGAACCT" "6-HU90-50pg-dp:GAGTGCT;GTTATCCT;ACAGCAGCT;TGAACATGCT" "7-G1-1ng-dp:AGGAGGT;AGTCTGGT;TCTGTACCT;CGACATACGT" "8-HU90-1ng-dp:CTATGGT;GACTTCCT;GGCACTGCT;GACTGTTCCT"

UNCLASSIFIED_pairCnt:	17944596
MISMATCH_pairCnt:	125138
1-G1-50pg-dp_pairCnt:	13709116
2-HU90-50pg-dp_pairCnt:	13045707
3-G1-1ng-dp_pairCnt:	15645664
4-HU90-1ng-dp_pairCnt:	15669425
5-G1-50pg-dp_pairCnt:	14193295
6-HU90-50pg-dp_pairCnt:	14123210
7-G1-1ng-dp_pairCnt:	13374350
8-HU90-1ng-dp_pairCnt:	13811323