### the original raw data: /seq/Illumina_runs/NextSeqData/NextSeqOutput/210622_NB501555_0948_AHWMH5AFX2/Data/Intensities/BaseCalls/308430 (105,376,295) In node bamdev1: enter the folder: /seq/Illumina_runs/NextSeqData/NextSeqOutput/211119_NB501555_1009_AHCN7MAFX3/Data/Intensities/BaseCalls/309509 scp *.* zwang@wigclust20:/mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-07_SC-seq-data_Elaine_NextSeq/. ### wait the copying finished... processed data: /mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-07_SC-seq-data_Elaine_NextSeq/ ### deconvolute the barcodes: /mnt/wigclust15/data/safe/leey/ArXiv/ToWhom/zihua/code/sfbc.differnet.barcodes.length/trimBarcodeFragments -nThreads=8 -inputFiles=/mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-07_SC-seq-data_Elaine_NextSeq/CE4_T3_NlaIII-NlaIII-TaiI_S1_R1_001.fastq.gz,/mnt/wigstore3/user/zwang/sequencingAnalysis/2021-12-07_SC-seq-data_Elaine_NextSeq/CE4_T3_NlaIII-NlaIII-TaiI_S1_R2_001.fastq.gz "1-NlaIII-dp:GTCAGCT;TCTCACCT;GGTATTCGT;AATTGATGCT" "2-NlaIII-ss:CACTCGT;CTAGACGT;ACGACAGCT;TGGCATACGT" "3-NlaIII+TaiI-dp:TCAAGCT;ATTGACGT;CAGATTCCT;TGTCGATAGT" "4-NlaIII+TaiI-ss:CGTTCGT;GCATAGGT;AAGGATGCT;GTCGTAAGCT" UNCLASSIFIED_pairCnt: 19428546 MISMATCH_pairCnt: 73474 1-NlaIII-dp_pairCnt: 32963220 2-NlaIII-ss_pairCnt: 42667018 3-NlaIII+TaiI-dp_pairCnt: 6856795 4-NlaIII+TaiI-ss_pairCnt: 3387242